Friday, April 15, 2016
Sperm DNA damage—the effect of stress and everyday life factors
M Radwan, J Jurewicz, D Merecz-Kot, W Sobala, P Radwan, M Bochenek, W Hanke. Sperm DNA damage—the effect of stress and everyday life factors. International Journal of Impotence Research. Advance online publication 14 April 2016; doi: 10.1038/ijir.2016.15.
The clinical significance of sperm DNA damage lies in its association with natural conception rates and also might have a serious consequence on developmental outcome of the newborn.
The aim of the present study is to determine whether stress and everyday life factors are associated with sperm DNA damage in adult men. The study population consisted of 286 men who attended the infertility clinic for diagnostic purposes and who had normal semen concentration of 20–300 m ml−1 or with slight oligozoospermia (semen concentration of 15–20 m ml−1) (WHO, 1999). Participants were interviewed and provided a semen sample. The sperm chromatin structure assay was assessed using flow cytometry.
In the present study, we found evidence for a relationship between sperm DNA damage parameters and everyday life factors. High and medium level of occupational stress and age increase DNA fragmentation index (P=0.03, P=0.004 and P=0.03, respectively). Other lifestyle factors that were positively associated with percentage of immature sperms (high DNA stainability index) included: obesity and cell phone use for more than 10 years (P=0.02 and P=0.04, respectively).
Our findings indicate that stress and lifestyle factor may affect sperm DNA damage. Data from the present study showed a significant effect of age, obesity, mobile phone radiation and occupational stress on sperm DNA damage. As DNA fragmentation represents an extremely important parameter indicative of infertility and potential outcome of assisted reproduction treatment, and most of the lifestyle factors are easily modifiable, the information about factors that may affect DNA damage are important.
... male sperm DNA damage measures: the percentages of DNA fragmentation index (DFI), medium DNA fragmentation index (M DFI), high DNA fragmentation index (H DFI) and high DNA stainability index (HDS—percentage of immature sperms).
Cell phone use was based on the years of usage of this equipment (0–5 years, 6–10 years, 11–25 years).
52.1% of study population used cell phone from 6 to 10 years.
Age category >40 years increased the H DFI (P=0.03). Obesity (BMI 30–40 kg m−2) and using cell phone more than 10 years was positively related to HDS (P=0.02 and P=0.04, respectively) (Table 2). Other examined lifestyle factors: smoking, alcohol consumption, coffee drinking were not related with any of the examined parameters of sperm DNA damage and high DNA stainability. The result were adjusted for potential confounders.
In our study, using a cell phone for more than 10 years increases HDS. A recent study showed that DNA fragmentation was the only parameter altered in mobile phone users, in a group of high usage (>4 h daily) that stored their phone in the trouser pocket.36 In addition, in the study performed in Poland using cell phone more than 10 years was negatively associated with the percentage of motile sperm cell.17
Although few studies have explored the association between exposure to stress and life factors, and male reproductive function, none have carefully assessed the percentage of medium DFI and the percentage of immature sperms. A detailed questionnaire information on demographics, medical, lifestyle risk factors performed among study participants allowed for control of confounding in the statistical models. The relative homogeneity of study participants (educated, white) helped reduce the chance that our findings resulted from unmeasured health, behavioral or exposure factors. This homogenicity increases the internal validity of our study, but limits the generalization of study findings to more diverse population. Additional strength arise from the fact that smoking status was verified using the level of cotinine in saliva.
In conclusion, our findings indicate that stress and lifestyle factor may affect sperm DNA damage. Data from the present study showed a significant effect of age, obesity, mobile phone radiation and occupational stress on sperm DNA damage. As DNA fragmentation represents an extremely important parameter indicative of infertility and potential outcome of assisted reproduction treatment and most of the lifestyle factors are easily modifiable, information about factors that may affect DNA damage are important.
Adams JA, Galloway TS, Mondal D, Esteves SC, Mathews F. Effect of mobile telephones on sperm quality: A systematic review and meta-analysis. Environment International. 70:106-112. September 2014. http://bit.ly/
For references regarding effects of exposure to cell phone radiation on male fertility see http://bit.ly/saferemrsperm
and on female fertility see http://bit.ly/femalefertility.
Joel M. Moskowitz, Ph.D., Director
Center for Family and Community Health
School of Public Health
University of California, Berkeley
Electromagnetic Radiation Safety
News Releases: http://pressroom.prlog.org/
Thursday, April 14, 2016
Stories From Parents About Their Children
"Headaches, insomnia, memory and concentration problems, anxiety, allergies, rashes are commonly reported.
Read these stories from parents. (Keep scrolling down for more)
Effect the Cell Towers Have Had on My Son
From A California Mother in the USA received July 2015
My son developed anxiety, OCD, insomnia, irrational fears, various neurological problems, personality changes immediately after the upgrade of an AT&T cell tower next to his school to 4G LTE technology, which was completed in June 2013. I didn’t even know there were cell towers next to his school until March 2015 because these cell towers were camouflaged as ball field light poles. (One was 300 ft away from the school, the other was 600 ft away)
His symptoms suddenly began when he started 4th grade in August 2013. He suddenly became fearful of fire drills, and they gave him anxiety attacks to the point where he had to be called out of class and into the principal’s office prior to the drills. He developed severe anxiety and a low threshold for stress. He couldn’t concentrate on his homework. He would stare at the instructions and not understand what he was supposed to do – I had to explain instructions to him and I had to help him. He could only work on homework for 20 minutes at a time before he became extremely frustrated and stressed. He became extremely forgetful, often forgetting his books and other materials needed for homework. He suddenly developed OCD and became paranoid about germs. He developed insomnia and could only sleep for 7-8 hours a night, whereas before he used to sleep at least 10. He cried daily over trivial matters, daily life became overwhelming for him, he often asked why he became sick suddenly and why he felt so stressed all the time, and he even began to say life would be better in Heaven because then he would no longer feel so stressed and sick. I was devastated to hear my son say these things.
He suddenly had behavioral problems, which his teachers never said he had before the 4th grade. When I had him undergo a psychological evaluation, it determined he had OCD, Anxiety, and ADD – he did not have any of these problems prior to the cell tower upgrade, except possibly for mild ADD that did not present as a problem. He also suddenly developed constipation, where he used to be regular. He developed unexplained rashes on the backs of his hands. His allergies went from mild to severe. His personality changed. He changed from being a sweet and thoughtful child to being angry and stressed most of the time who had a hard time functioning in school. Simple tasks took him twice as long to do – getting ready in the morning took him 1.5 hrs where it used to take him 45 min. He became a different person and I was terrified I was losing him to some mysterious illness. Fourth grade was a terrible year for him and for us as a family – it took a tremendous amount of effort just to get through each day one day at a time.
His pediatrician ran extensive tests on him and could not find anything wrong physiologically and ruled out any diseases. We began all kinds of treatments to try to get him back to normal, including neurofeedback, acupuncture, nutritional supplements, herbal medicine. He began to see many new medical practitioners that he didn’t have before, including pediatric GI specialists, psychologists, psychiatrists, acupuncturists, allergists, and a neurofeedback specialist. He had at least 2 medical appointments a week! In March 2014, after 6 months of hard work and thousands of dollars spent on these various therapies, he began to return to normal and he was able to function normally again. It was also during this time that we instituted a 504 (special ed) for him for the first time. No one knew what was wrong, and I didn’t make the connection that it was due to the cell towers next to the school until I discovered them in March 2015.
By June 2014 (after 10 months of therapy) he was almost back to himself, and he was functioning normally throughout the 5th grade until April 2015. His OCD was gone, his anxiety levels were down to a manageable level, and his ADD was mild and manageable. He became a happy, normal child again, and I was grateful to have him back to himself, even though he still needed to continue with his various therapies to keep his symptoms under control.
Then in March 2015 I saw workers working on the ball field light poles – the covers were off, and I saw cell antennas - that’s when I discovered there were cell towers next to the school. I asked them what they were doing, and they said they were upgrading the T-Mobile tower to 4G LTE. I was shocked there was a cell tower so close to school (300ft). So I went to City Hall to investigate, and that’s when I discovered the presence of a second cell tower (AT&T) next to the school at 600 ft, and that it had been upgraded to LTE in June 2013! That’s when I realized what triggered my son’s sudden anxiety and severe allergy problems in August 2013.
So I bought an RF meter to measure the RF power densities around the school, and I found that they were at a maximum of 0.3uW/cm2 outside. So I researched to see what kinds of symptoms could be expected at these levels. That’s when I found the 2012 Bioinitiative Report. At the RF levels measured, it was high enough to increase the risk of leukemia by a factor of 2, increase headaches, insomnia, memory and concentration problems, stress hormones and anxiety, behavioral issues, and rashes according to the 2012 Bioinitiative Report. When I saw that the symptoms my son had matched what was in the 2012 Bioinitiative Report for the RF power densities measured at his school, that was further confirmation of what triggered my son’s anxiety and severe allergy problems. That’s when I started taking action.
Within a few days that the second cell tower (T-Mobile) was upgraded to 4G LTE in April 2015, he began showing the same signs again – extreme allergies to he point that he’s developed asthma now (1st time), OCD reappearing, anxiety escalating. His younger brother who is 6 years old has started to show some similar symptoms that he had not before. His younger brother has been having GI problems, allergies (never had allergies before), and changes in mood (emotions). As a parent, having seen him go through these changes twice immediately after the cell tower upgrades, I have to do something. This could not have been a coincidence. These are just the effects I am seeing on the outside. What is going on inside of him? This must be affecting other kids too. That’s when I started working on setting safer cell tower setbacks from schools – 1500ft, which is the usual recommended standard based on scientific studies.
That’s my story. Like most people, I had no idea that cell towers could have such a profound effect on one’s health and well-being. I have a B.S. degree in Chemical and Bio Engineering, and it was hard for me to accept initially that RF radiation could have been the cause of my son’s illness. However after doing much research on the subject (including Andrew Goldsworthy’s papers) and experiencing what I did firsthand, it was the only rational conclusion.
I hope you will share your stories too if you or a family member has been affected by cell tower radiation. By publicizing our stories we will bring awareness to this issue and force our government (FCC) to change its outdated regulations on safe levels of RF exposure from 1996.
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